Flawed Fish Jaws Shed Light on Hearing Loss in Humans

Genetic Mutation – Malformation of Jaw

As per USC research in Scientific reports, the same genetic tweak which tends to cause malformed jaws in the case of fish could be responsible for some issues in hearing in humans which seems to have some evolutionary origins. Scientists are of the belief that the arrangements which seem to support the jaws of primeval ancestral fish gave rise to three tiny bones towards the middle ear of humans as well as other mammals which transmit sound vibrations where the bones are known as malleus, incus and stapes.

 In zebra fish, a genetic mutation could result in malformation of the jaw and hence USC researchers speculated if an equivalent genetic change could activate hearing defects in mice and humans. To comprehend the query, Camilla Teng, USC PhD student coordinated along with the other colleagues in the USC Stem Cell laboratories of Gage Crump, Rob Maxson, and Neil Segil and with clinical experts in radiology, audiology and genetics at the Keck School of Medicine of USC and Children’s Hospital Los Angeles.

They researched on two genes JAG1 and NOTCH2 which were mutated in most patients with Alagille syndrome - AGS which a genetic condition causing several symptoms in various parts of the body inclusive of the liver.

Hearing Loss – Deficits in Sensory Cells of Inner Ear

A person with Alagille syndrome tends to have less than normal number or small bile ducts in the liver which is the organ in the abdomen between the chest and hips that makes blood proteins, bile storing energy and nutrients, combats infection as well as eliminates harmful chemicals from the blood.At least in half of the patients, the syndrome tends to affect hearing loss besides liver, eye, heart and skeletal defects.

Though some of this could be hearing loss due to deficits in the sensory cells of the inner ear, the researchers have been speculating on the conductive hearing loss that involves essential components of the middle ear like the vibrating bones.

With the introduction of mutations in mice, the researchers observed flaws in the incus as well as the stapes bones together with corresponding hearing loss. Thereafter they attended Alagille Alliance meetings in 2011 and 2014 performing hearing tests later on 44 human patients affected with Alagille syndrome to determine if their hearing loss had been conductive, sensor in neural or mixed.

Conductive Hearing Loss

As predicted by their discoveries in zebra-fish and mice, they observed conductive hearing loss had been the most common type which had affected almost one third of all ears. The CT scans of 5 AGS patients showed a more considerable complex picture, an unexpected variety of basi cflaws in the middle ear having variable effects on hearing.

One out of the five patients had a stapes flaw precisely related with conductive hearing loss.Teng commented that their study emphasized a generally unnoticed phenotype of Alagille Syndromes.

According to Teng, the study provided some insight on generally overlooked issue arising among individuals with Alagille syndrome. She stated that if patients tend to be conscious of possible conductive hearing loss earlier in life, they can seek medical aidin time for abetter quality of life.

Peering Into Fish Brains to See How They Work

Transparent Fish – Work in the Dark

The main focus in the research of the latest group at the Kavli Institute for Systems Neuroscience is transparent fish and the capability to work in the dark. One of the important challenges faced by neuroscientists wanting to comprehend how the brain works is essentially reckoning out how the brain is wired together and how neurons tend to interact.

NTNU neuroscientists and Nobel laureates May-Britt and Edvard Moser resolved this issue by studying how to record from individual neurons in the rat brain when the rats tend to move freely in space. They utilised the recording in order to make the findings that had attained them the Nobel Prize.

They were in a position to understand that certain neurons in the entorhinal cortex fired in a manner that created a grid pattern which could be utilised in navigating like an internal GPS. Emre Yaksi, the latest teamhead of the Kavli Institute for Systems Neuroscience utilised a diverse approach to the issue of viewing what tends to go on within the brain.

Rather than studying rats or mice, Yakshi resorted to around 90 various types of genetically modified zebra-fish which he could breed in creating various fish with preferredphysiognomies.

Comprehending Universal Circuit Architectures in Brain

Young larval zebra-fish are said to be totally transparent and hence Yakshi needed only a systematic optical microscope to view what tends to occur inside their heads. Some of the fishes of Yakshi seem to have a genetic modification which makes their neurons light up while they direct signal to another neuron and he has informed that this is what tends to make circuits and connections visible to researchers.

He commented that they are interested in comprehending the universal circuit architectures in the brain which can perform interesting computation. Though fish are quite different from humans, their brains tend to have identical structures and in the end fish also have to find food, they also have to find a mate, they have to avoid dangers and they build brain circuits which can generate all these behaviours just the way humans tend to do.

When Yaksi had come to Kavli Institute in early 2015 together with a team of researchers they had a 900 kg anti-vibration table which was the size of a billiards table. The table had been big and heavy and was needed in the laboratory to reduce vibration when they had to use the highly sensitive optical microscopes to peer into the brains of the zebra-fish.

Zebra-Fish Genetically Adapted

The larval fish tend to be quite small that a slight vibration from cars or trucks passing by the streets could make the microscopes bounce away from their miniature brain targets. Zebra-fish brains are quite small, around 10,000 to 20,000 neurons which is a figure dwarfed by the human brain that tends to have an estimated neurons of 80 billion.

However the measurement that Yaksi together with his colleagues tend to make marks in huge quantities of data. According to him, a 30 minute of recording could generate data which tends to take about a week to process the same. It was for this purpose, the research group of Yaksiis a multi-disciplinary team of engineers, physicists and life scientists who seemed to be trained to develop and utilise computational tools in analysing these huge datasets.

Since few of the zebra-fish tend to be genetically adapted in order that their neurons light up with a fluorescent protein when the neurons are active, Yaksi and his colleagues tend to work frequently in low light or darkness. This is particularly obvious when he takes visitors in the subdued darkness of the laboratory where several of the fanciest microscopes are confined in boxes open towards the front, developed to restrict the amount of external light.

Research – Causes of Seizures/How Seizures Prevented

Yaksi had informed that other zebra-fish are genetically modified to shine a blue light in their brain which tends to activate certain neurons enabling the researchers to plan connections between neuron. Major part of the study being done by the group of Yaksiis basic research with findings which tend to improve our understanding of the brain computation though does not specifically have any instant clinical implications.

However, Nathalie Jurisch-Yaksi, wife and colleague of Yaksi is working with medical doctors in order to develop genetically modified zebra-fish which could be helpful in shedding light on brain disease like epilepsy.According to Yaksi, most of the people in his lab are doing basis research attempting to ask how does the brain works, how is it connected, how is it built.

 Nonetheless, Nathalie is working at NTNU with medical doctors and they are trying to reach out to clinicians. For instance he stated that if a brain disorder like epilepsy tends to have a genetic component, that same genetic mutation could be developed in the transgenic group of zebra-fish facility in order that the team could research on the causes of seizures in a diseased brain and how the seizures can be prevented.

Kavli Institute – Excellent Science Environment

The Kavli Institute had been on an institute-wider retreat, when he had come to Trondheim for interview for the position, so Yaksi had the opportunity of meeting not just group leaders but also technicians, master’s students, PhD candidates and everyone. He informed that what was most impressive besides the excellent science environment was that people had been happy and satisfied with what was being done and it was a good atmosphere.

 Though the science had been the most serious part of his decision to move to Trondheim, he informed that he was excited to be a part of the Kavli Institute since he and his wife desired to live in a smaller town as well as close to nature.

He had stated that Trondheim seems to be a unique place and one can do really good science and yet be close to nature, which was a big thing for him and his wife. Going to London or another big city was never an option and they did not desire to deal with big city life. He also informed that when May-Britt Moser had asked him at the time of his interview on what he knew regarding Scandinavia. His reply had been that he did not know much though he had added that he and his wife loved being outdoors.

Plastic 12-Bit RFID Tag and Read-Out System With Screen-Printed Antenna

Quad Industries, Agfa, Imec and TNO made an announcement recently that they established and verified a plastic 12-bit RFID tag and read-out systems with security that is screen printed. For the first time, the system combines a screen-printed antenna and a printed user interface that is based on touch, which allows the reader to operate on curved surfaces. The demonstrator has developed for applications pertaining to badge security, but also shows scope for many other applications as well such as smart packages, games that require interaction and wearables.

Compared to silicon (Si)-based identification devices, RFID tags that are made of plastic electronics have more advantages. They can be attached to curved packaging, effortlessly incorporated in everyday objects and its manufacturing is low-cost. The usual application consists of identification of items, smart food packaging, protecting the brand and badge security. A dedicated RFID reader is needed to scan the RFID tag which is usually in two centimetres of the tag. The antenna in the tag as well as the reader should both be flexible, utilising the advantages of plastic electronics to the fullest. Screen-printed antennas have been applied effectively on the top of an RFID tag but inflexible PCB-based antennas are generally used by the read-out systems. This is primarily because of the fact that the printed antenna has a poor resistance and Q-factor.

For the first time, industries like Imec, Quad Industries and Agfa have combined a screen-printed antenna in both of the items, the RFID tag as well as the read-out system. This allows the application of both these devices on a diverse range of surfaces. Quad Industries have screen-printed antennas using printing inks from Agfa.

This new technology has been demonstrated in an application pertaining to badge security. The access badge integrates the printed antenna, which is size of a credit-card, with a plastic 12-bit RFID chip, placed on plastic substrate that’s flexible. Imec’s metal-oxide thin-film transistor (TFT) technology has been used to manufacture the RFID tag. Large-area manufacturing processes are used by this technology that makes large-scale production at a low cost possible.

The read-out system includes uniquely printed functionality at diverse levels. To begin with, an RFID read-out antenna is made by screen-printing on a plastic film, making room for best possible integration on flat, curved or 3-D shaped reading surfaces. Also, a fully printed touch screen interface with numerical keypad has been placed between the cover lens and the display, which allows any user without a badge to enter the building by punching in a numerical code. Highly transparent screen-printed inks have been used to print these printed touch screen.

There are recently developed Ag inks which are nanoparticle based that makes lower resistances over conventional Ag-flake based inks achievable which in turn enables integrating new functionalities directly by screen printing. In addition to this, the antenna is printed at the same level as the printed touch screen which results in direct, more economical combination of the printed antenna and the customized touch screen in the device that’s the reader.

This technology allows for economical screen-printing manufacturing, is effortlessly customizable and eco-friendly and allows direct chip integration on many substrates which includes plastics, paper, etc. This technology also sees a promising use in smart packaging, smart PCB and smart gaming.

Sensor Solution: Sensor Boutique for Early Adopters

It is known that a very individual fraction of infrared light is absorbed by every chemical substance. This absorption can be used for recognising substances with the help of optical methods, which is almost like the concept of a human fingerprint.

To elaborate this concept, when the infrared radiation, that falls within a certain range of wavelength, are absorbed by molecules, they are animated to a higher level of vibration, in which they rotate and vibrate in a typical and distinctive pattern or rather in a “fingerprint” pattern. These patterns can be used for identifying specific chemical species. Such kind of a method is used, let’s say, for example, in the chemical industry but also has its uses in the health sector or in criminal investigation. A company often needs an individually tailored sensor solution if it plans a new project.

EU-funded pilot line called MIRPHAB (Mid InfraRedPhotonics devices fABrication for chemical sensing and spectroscopic applications) support companies that in search for a suitable system and help in the development of sensor technology and measurement technology in mid-infrared (MIR). Participating in this project is the Fraunhofer Institute for Applied Solid State Physics IAF.

Pilot line for ideal spectroscopy solutions

A company has very individual needs if it is looking for a sensor solution, for example, if it has to identify a particular substance in a production process. This begins with the substances that have to be recorded to the number of sensors required up to the speed of the process of production.Considering most of the cases, a custom-made solution that suits all does not suffice and various suppliers are required for the purpose of developing the optimal individual solution.Here is where MIRPHAB comes into picture and proves to be very useful.

Leading European research institutes and companies belonging to the MIR environment have collaborated to provide customers with a custom-made and best suited offers made from a single source. Parties that are interested can get in touch with a central contact person, who can then make a compilation of the best solutions possible from the MIRPHAB members component portfolio as per the modular principle.

EU funding has supported MIRPHAB in the development of the individual MIR sensor solution within the framework, in order to fortify the European industry in the long run and increase in its leading position in chemical analysis and sensor technology. This considerably lessens the investment costs and as a result also reduces the entry point for companies in the MIR area.

Companies that have previously faced high costs and development efforts are now looking at a high-quality MIR sensor solution as an object of interest due to its combination with the virtual infrastructure which is a development caused by MIRPHAB.Also, MIRPHAB provides companies access to the latest and modern technologies, enabling them with an added advantage as an early adopter compared to the competition.

Custom-madesource forMIR lasers

The Freiburg-basedFraunhofer Institute for Applied Solid State Physics IAF along with the Fraunhofer Institute for Photonic Microsystems IPMS situated in Dresden, is providing a central component of the MIRPHAB sensor solution. The Fraunhofer IAF is presenting the new technology of quantum cascade lasers that emanate laser light in the range of MIR. In this type of laser, the range of the wavelength of the emitted light is spectrally extensive and can be adapted as per requirement during manufacturing. To select a particular wavelength within the broad spectral range, an optical diffraction grating has to be used to choose and then coupled back into the laser chip. The wavelength can be adjusted constantly by turning the grating. This grating is created at the Fraunhofer IPMS in a scaled-down form in so-called Micro-Electro-Mechanical-System or MEMS technology.Thus it is then possible to oscillate the grating up to one kilohertz of frequency. This further enables the tuning of the laser source’s wavelength up to a thousand times per second over a large range of spectrum.
The Fraunhofer Institute for Production Technology IPT in Aachen also has involvement in MIRPHAB in order to make the manufacturing of lasers and ratings more proficient and to enhance them for pilot series fabrication.With the help of its proficiency, it changes the production of the quickly adaptable MIR laser into industrially applicable manufacturing processes.

Process exploration in actuality

Currently, there are many applications in the field of spectroscopy that are still in the category of visible or near the range of infrared and use comparatively feeble light sources. MIRPHAB provides solutions has the concept of infrared semiconductor lasers as a foundation. These have comparatively higher intensity of light thus allowing the scope for completely new applications. This results in a recording of up to 1,000 spectra per second with the help of the MIR laser source which, as an example, provides for the real time programmed monitoring and control of biotechnological processes and chemical reactions. Thus, MIRPHAB’s contribution is considered to be important and vital to the factory of the future.

Can Artificial Intelligence Help Us Make More Human Decisions?

About 88 million pages of original and authentic handwritten documents belonging to the past three-and-a-half centuries, line the tiled halls of a simple 16th-century trading house located right in the middle of Seville, Spain. These are stored here, incompletely transliterated, where some of them are almost indecipherable. A few of them were carried back on armadas from the Americas while a few have undergone scanning and digitisation.

These documents contain the answers and the context for the innumerable questions pertaining to the Conquistadors, the European history, the New World contact and colonialism, politics, law, economics and ancestry. However, it is unfortunate that hardly some of these carefully kept pages were ever read or interpreted since they were written and brought to Seville centuries before and it is highly unlikely that most of them never will be.

All hope is not lost as a researcher from the Stevens Institute of Technology is trying to get computers to read these documents, before we are out of time, while the documents are still readable. A Stevens computer science professor, Fernando Perez-Cruz asks “What if there was a machine, or a software, that could transcribe all of the documents?”.

Perez-Cruz, who’s expertise lies in the research area of machine learning also says “What if there was a way to teach another machine to combine into groups those 88 million pages and convert them into searchable text categorised into topics? Then we can start understanding the themes in those documents and then will be aware where to look in this storehouse of documents for our answers”. Thus Perez-Cruz is working on both factors of this two-fold approach which, if right, could then be applicable to many other new age and futuristic data analysis queries such as independent transport and analysis of medical data.

Pricing on Amazon, medical study, text reading machines

Perez-Cruz, who is a veteran of Amazon, Bell Labs, Princeton University and University Carlos III of Madrid, has had a very interesting career dealing with scientific challenges.In 2016, he joined Stevens and contributed to the growing asset of the computer science department of the university. Stevens aims at making this a strong research department which in turn is drawing more talent and resources. Perez-Cruz is using this to his advantage in his work. Currently, at Stevens, he is working to develop something called as ‘interpretable machine learning’ which is a systematized intelligence that humans can still work on.

As far as the problem of the historical document analysis is concerned, Perez-Cruz is in the hopes that he will be able to develop improved character-recognition engines. With the help of short excerpts of documents written in varied styles, which have been earlier transliterated by experts, he aims to teach software to identify both the forms of characters and often correlated associations between letters and words, thus constructing a growing recognition engine over time that is absolutely precise. The only question remains, he says, is that how much data or how much handwriting that is transcribed, is sufficient to do this well. The work on this concept is still developing.

Perez-Cruz states that he believes even though it is a technical challenge, it may still be achievable. He is even more fascinated about the next part which is organisation of large quantities of transcribed matter into topics that can be used in a glance. He says that the machine should be able to give us information right away from these three-and-a-half centuries of data when transcribed and should itself learn from the locations of the words and sentences. This is, what he calls, topic modelling.

A key link: Systematically grouping large data into easily accessible topics

After sufficient data has been entered into the algorithm, it begins to spot the most vital identifying and organizing forms and designs in the data. Very often, it so happens that various cues from the human researchers are vital and are searched for.Perez-Cruz notes that eventually, we might discover that there are, let’s say, a few hundred topics or descriptions that run through the whole of this archive and then all of a sudden there may be 88-million-document problems that have been scaled-down to 200 or 300 ideas.

If algorithms can consolidate 88 million pages of text into a few hundred lots, a huge progress in systematisation and efficiency can be achieved by historians and researchers who need to make choices about which particular document, theme or time periods are to be searched, reviewed and analysed in the formerly unmanageable archive. The same concept could be used to find styles, themes and concealed meaning in other vast unread databases.

He concludes saying that one begins with a huge quantity of unorganised data and in order to understand what material does that data contain and how it can be used, a kind of a structure needs to be brought to that data. Once the data is comprehended, one can begin to read it in a particular way, understand better what questions are to be asked pertaining to that information and make better conclusions.